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East Nusa Tenggara Province is a malaria endemic area located in Eastern part of Indonesia with Annual Parasite Incidence of 7.04% including falciparum malaria, vivax malaria and mix malaria. The information on parasite density and plasma levels of TNFα and IL-10 in mix malaria infection is rarely found. This research is conducted to collect the information mentioned above. Methods: Diagnosis of malaria infection in subjects was done by Rapid Diagnostic Test (RDT) as well as microscopy examination. Parasite density was calculated based on number of parasite per 200 leukocytes on the Giemsa-stained thick blood films. Levels of TNF-α and IL-10 in plasma were measured using Enzyme-Linked Immunosorbent Assay (ELISA). Results: Five patiensts were diagnosed as mix malaria infection, with average of parasite density was 4341 paracite/μL. Average level of TNF-α and IL-10 was 207.31 pg/mL and = 15.91 pg/mL, respectively. Ratio of TNF-α : IL-10 was 13 : 1. This research concludes, increased levels of TNF-α will decrease the parasite density based on the time of infection, while the increase in parasite density is directly proportional to elevate levels of IL-10 (p= 0,032).

proceedings of the 2nd international conference postgraduate school

Correlation between Parasite Density with Plasma Levels of TNF-α and IL-10 in Malaria Mix Infection in East Sumba District, East Nusa Tenggara Province