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In this contribution we present a compact imaging system to monitor the proliferation and the motility of cells in real-time. Our monitoring system is compatible with standard multi-well plates and operates in CO2, temperature and humidity controlled cell-culture incubators. Adherent grown epithelial cells in a multi-well plate well, positioned on top of a custom made holder, have been monitored in real-time with a fixed CCD image sensor. As light source an LED is placed above the plate holder. A field of view of 3.3 × 2.5 mm was achieved by using a 4.6 × 4.0 mm image sensor and mini lens system. The image sensor has a resolution of 640 × 480 pixels. Consequently, the obtained sensing resolution of the imaging system is about 5 μm. The cell monitoring system has first been validated by visualizing micro-beads of known dimensions. Then, our system has been successfully tested, tracking the migration paths and proliferation of respectively adherent grown MDCK (Madin-Darby canine kidney) and A549 (human lung carcinoma) epithelial cells.

A REAL-TIME CELL PROLIFERATION AND MOTILITY MONITORING SYSTEM