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To circumvent the obvious waste of resources when producing good marbled beef, research is needed to clarifymechanisms which are involved in intramuscular fat storage in cattle. The possible association between theactivity of diacylglycerol acyltransferase (DGAT) and muscle fat content was examined in samples oflongissimus dorsi (LD) and semitendinosus (ST) muscle from Holstein and Charolais bulls. The Holstein bullsexhibited higher fat content in both muscles and higher marbling score. In Holstein, DGAT activity wasenhanced in the LD muscle, and there was a tentative positive relationship between DGAT activity and the fatcontent in ST muscle. When muscle DGAT activity was examined as a function of DGAT1 genotype for allanimals, regardless of breed, the DGAT activity of LD muscle of the K/K genotype was about five-fold greaterthan for either the K/A or A/A genotypes. Further investigation on the relationship between DGAT1 genotypeand i.m. fat is required before this may be developed as a selection tool for marbling

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Diacylglycerol acyltransferase (DGAT) activity in relation to muscle fat content and &amp;lt;i&amp;gt;DGAT1&amp;lt;/i&amp;gt; genotype in two different breeds of &amp;lt;i&amp;gt;Bos Taurus&amp;lt;/i&amp;gt; (short communication)