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As the use of antibiotics as growth promoters have been banned in different countries, researchers have been working hard to find alternatives to antibiotics, and also come up with other substances that can promote growth, health and productivity in broiler chickens. The study was conducted to evaluate the serum biochemistry of broiler chickens served dietary inclusion of probiotics (Saccharomyces cereviasae) and enzyme. One hundred and twenty, one day-old broiler chicks Ross strain were used for the study. The birds were randomly allotted to four dietary treatments. Each treatment contained 30 birds replicated three times to contain 10 birds per replicate in a completely randomized design experiment. The four dietary treatments were T1 Control (no probiotics and no enzyme), T2 (both probiotics and enzyme), T3 (Probiotics only) and T4 (Enzyme only). The probiotics and enzyme was included at 0.8% and 0.02%, respectively. The birds were given feed and water ad-libitum. The experiment lasted for eight weeks. At the end of the experimental period, three birds were randomly chosen from each replicate for serum biochemistry profiling and liver function test. The results showed that inclusion of probiotics and enzyme had significant effect (p<0.05) on the serum biochemistry profile and liver function indices. Low density lipoprotein was reduced from 3.83±0.60g/l in the control group to 2.18±0.12g/l in T2 (Probiotic and Enzyme) group. Lymphocytes values ranged from 26.00% to 38.00% in the treated groups, while the control had 45.67%. The dietary inclusion of probiotics and enzyme reduced total cholesterol from 5.07g/l to 4.22g/l and low density lipoprotein from 3.83g/l to 2.18g/l in the (probiotics and enzyme) treated group and also improved liver function indices of the broiler chickens. Aspartate aminotransferase, Alanine aminotransferase, Alkaline phosphatase and total bilirubin had range values of 114.67 to 150.67 μl, (21.00 to 28.67 μl, 10.33 to 17.00 μl and 1.30 to 1.47mg/dl, respectively. It was therefore, concluded that combination of probiotics and enzyme should be included in broiler diets for immunity improvement, good liver function and reduction in cholesterol levels.

Serum biochemistry profile and liver function indices of broiler chickens served dietary inclusion of probiotics (Saccharomyces cerevisiae) and enzyme