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Climate change has an impact on agriculture and insect pests that affect the crop production. Aphid pests occur throughout the temperate region of the world andcause direct damage by sucking plants sap which affects growth and yield of the crops. Species of aphids are diverse and ubiquitous but their importance among crop differs.Ultraviolet radiation (UVR) is an electromagnetic radiation and constitutes about 10% of the total light output of sunlight. Ultraviolet-B coming due to ozone depletion is the most photo chemically reactive wavelength of solar radiation and important stressor to organisms particularly in their early stages of life and affect ecosystem. Psoralene and alphaterthienyl are naturally occurring plant metabolites found in many crops and become photo reactive in presence of solar ultravioletB radiation. Measurement of solar ultraviolet radiation (UV-B and UV-E) was performed in Garhwalregion of Uttarakhand. Morphological, behavioural changes, mortality rate and glutathione contents were observed in mustard and roseaphids after ultraviolet-B irradiation with psoralene and α-terthienyl photo sensitizers. UV-B and UV-E radiation showed seasonal, diurnal and altitudinal variations. αterthienyland psoralenewith ultraviolet-B radiation showed stunted growth, morphological changes, low reproduction and high mortality up to 26% in aphids. Reduction in glutathione (GSH) level in aphids was also observed after UVB and photosensitizersexposure.Enhanced UV-B was found more toxic with photosensitizers andthus, can be used as natural insecticide. Dose, intensity and species dependent changes in toxicity of psoralene and αterthienyl were observed in aphids. Resultsindicate that UV-B radiation with photosensitizers has toxic effect on aphids population, dispersal and diversity. Psoralene was found more toxic than αterthienyl in aphids. Mustard aphid (Lipaphis erysimi) was found more sensitive than rose aphid (Macrosiphum rosae).

PHOTOTOXICITY OF α-TERTHIENYL AND PSORALENE INROSE AND MUSTARD APHIDS (MACROSIPHUM ROSAE & LIPAPHIS ERYSIMI) IN DOON VALLEY