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Docosahexaenoic acid (DHA) supplementation has been reported to negatively correlate with cancer cell proliferation and tumourdevelopment in many cancer types. Although cumulative evidence hasdemonstrated the apoptotic effect and cytotoxicity of DHA against tumourdevelopment in many cell types, the precise cellular and biochemical mechanisms of DHA-induced apoptosis in human endometrial cancer cells havenot been investigated.    Material and methods  MTT assay was performed to confirm the degree ofapoptosis by combining treatment with DHA and triacsin C in endometrialcancer cell line. The synergistic effects of triacsin C and DHA were identifiedby performing flowcytometry and immunoblotting analysis.    Results  Combined treatment with DHA and triacsin C significantly inducedapoptosis in RL95-2 endometrial carcinoma cells. Combined treatment with125 μM DHA and 5 μM triacsin C significantly increased the sub-G1 population and apoptotic fragments in endometrial carcinoma cells. It was alsodemonstrated that DHA and triacsin C induced apoptosis through mitochondrial pathways via caspases-9, -3, and -7 as well as through the extrinsicpathway by activation of caspase-8/BID.    Conclusions  Further elucidation of the apoptotic mechanisms involvingDHA treatment with ACS ablation could shed light on possible new treatment strategies for endometrial cancer. In addition, further research intothe mechanisms of DHA and triacsin C-induced apoptotic mechanisms maylead to the development of therapeutic strategies for endometrial cancer.

Induction of apoptosis in RL95-2 human endometrial cancer cells by combination treatment with docosahexaenoic acid and triacsin C