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Natural products are valuable and well known for their biological activities. In the current scenario, the research and analysis of plant leaf extract and nanoparticles synthesis with their biological activities has been expended significantly. For the synthesis of nanoparticles, Copper is the preferred metal among other metals due to its reported use in medical field as antimicrobial agents and its lethality. The aim of the present work is to assess antimicrobial and antioxidant activities of the methanolic leaf extracts and aqueous leaf extract mediated copper nanoparticles of Parthenium hysterophorus. Study also revealed the comparative analysis between methanolic leaf extracts and copper nanoparticles. Synthesis of copper nanoparticles (CuNPs) was confirmed by the change of color of aqueous extract, which were further confirmed by using UV-Vis spectrophotometer. The results indicated that copper nanoparticles have great antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Candida albicans and Aspergillus flavus rather than methanolic leaf extract. These studies confirmed that copper nanoparticles are capable to rendering high antimicrobial efficacy and hence has a great potential in the development of antimicrobial agents. Based on the DPPH, copper nanoparticles found to be good antioxidant when compared to methanolic leaf extract. Furthermore cells viability assay was also done against copper nanoparticles. These results concluded that copper nanoparticles are good source of therapeutic agent and applications of copper nanoparticles based on these findings may lead to valuable discoveries in various fields such as medical devices and antimicrobial systems.

Antimicrobial, Antioxidant and Cytotoxic Activity of Green Synthesized Copper Nanoparticle of Parthenium Hysterophorus L.