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espanolObjetivos: Evaluar el papel de la enzima antioxidante catalasa sobre el proceso de calcificacion vascular asociada a insuficiencia renal cronica (IRC) y su efecto sobre la masa osea. Material y metodos: Se utilizaron ratones C57/BL6J salvajes (WT) y transgenicos (TG), que sobreexpresan la enzima catalasa, a los que se les indujo IRC. Se utilizaron como control ratones WT y TG con intervencion simulada. Transcurridas 16 semanas los animales se sacrificaron, obteniendo suero para analizar marcadores bioquimicos, el trozo residual de rinon, la aorta y las tibias. Se utilizo igualmente un modelo in vitro de cultivo primario de celulas de musculo liso vascular (CMLV) procedentes de aorta de raton WT y TG sometidas durante 8 dias a un medio calcificante con 3 mM de fosforo y 2 mM de calcio. Resultados: Solo en animales WT con IRC se observo un incremento significativo en la expresion genica de Runx2 y del deposito renal de calcio y un deterioro de la estructura osea a nivel trabecular. Este efecto no se observo en ratones TG con IRC. Solo en las CMLV de ratones WT, la adicion de medio calcificante produjo un aumento del contenido en calcio, de la expresion proteica de Runx2 y de las especies reactivas de oxigeno mitocondriales con una menor expresion proteica de la enzima catalasa. Conclusiones: La sobreexpresion de la enzima catalasa redujo el proceso de calcificacion tanto in vivo como in vitro, mostrando in vivo que ese descenso se acompano de una mejora en los parametros oseos estudiados. EnglishObjetives: Assess the role of the catalase antioxidant enzyme in the vascular calcification process associated with chronic renal failure (CRF) and its effect on bone mass. Material and methods: Wild type C57/BL6J mice (WT) and transgenic mice (TG) were used, that overexpress the catalase enzyme, to which CRF was induced. Control WT and TG mice were used in simulated intervention. After 16 weeks, the mice were sacrificed, with serum samples taken for biochemical markers as well as residual pieces of kidney, aorta and tibias. An in vitro model of primary culture of smooth vascular muscle cells (SVMC) taken from the WT and TG aorta which underwent eight days of 3 mM phosphorus and 2 mM calcium calcifying medium. Results: A significant increase in Runx2 gene expression, calcium renal deposit and bone structure deterioration at trabecular level was only detected in WT mice with CRF. This was not observed in TG mice with CRF. Only in the case of WT mice SVMC, did added calcification medium raise calcium levels, proteic Runx2 expression and the reactive oxygen species of mitochondria with low catalase enzyme. Conclusions: Calcifying catalase over-expression was observed in both in vivo and in vitro, with in vivo showing that this reduction was accompanied by an improvement in bone parameters under study.

Efecto de la enzima antioxidante catalasa en la calcificación vascular y desmineralización ósea