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Aim: To evaluate the In vitro efficacy of a new biologically acceptable Anti Microbial-Solution (AMS) for prevention of colonization of polymeric denture base materials. Materials and methods: Sample discs of 6.25mm in diameter and 2mm thick were sectioned from molded rods using two types of Poly Methylmethacrylate resins (heat and chemical polymerizing). Rate of absorption for the different sample discs was determined for water and for AMS. The concentrations of AMS effective against Streptococcus mutans, Lactobacillus paracasei, Actinomyces naeslundii, Prevotella intermedia, Enterococcus faecalis and Candida albicans were determined and scanning electron microscopy (SEM) was used to confirm colonization of samples. Antimicrobial efficacy of absorbed AMSin the resins was also evaluated. Results: The absorption rate of AMS and water was the same and maximum absorption of about 1% was reached after 48h of soaking. SEM confirmed that the organisms were able to colonize the material surfaces regardless of type of material. AMS was more effective against anaerobic organisms and lesseffective against aerobic organisms and inhibition of growth of organisms by AMS started at 10% concentration. Although inhibition was observed with absorbed AMS, no consistent inhibition pattern was found. Conclusion: Efficacy of AMS in the prevention of micro-flora colonization of denture-base materials is reported and AMS is shown to be more effective against anaerobic than aerobic organisms. Lack of optimization of the absorptioncoefficient of the polymeric resin and the determination of effective concentration of AMS may have led to inconsistent inhibition patterns. Keywords : In vitro , Prevention, Micro-flora Colonization, Denture-base Materials Tanzania Dental Journal Vol. 14 (1) 2007: pp. 15-25

<i>In vitro</i> efficacy of an anti-microbial solution in prevention of micro-flora colonization of denture-base materials