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Les premieres etudes sur les mecanismes de defense du palmier a huile ont mis en evidence le role des composes phenoliques dans la tolerance a la fusariose des clones et croisements a partir des racines inoculees par l’agent pathogene. Notre travail a consiste d’une part a ameliorer les premieres techniques d’extraction en remplacant l’Ethanol par un melange Ethanol-eau et d’autre part a ameliorer les techniques de microanalyses des marqueurs de tolerance a la fusariose. Les resultats ont montre que les modifications apportees ont permis d’acceder a une quantite plus importante de phenols que la methode classique. En outre, les analyses des phenols totaux et la fongitoxicite ont permis de discriminer les clones tolerants des clones sensibles. Ces nouvelles methodes nous ont permis d’evaluer plus rapidement la tolerance a la fusariose des clones apres inoculation de l’agent pathogene. The early studies on the defence mechanisms of the oil palm tree put in evidence the role of phenolic compounds in the tolerance of wilt disease of the clones and crossings from the roots inoculated by the pathogenic agent. Our work consists on one hand in improving the first techniques of extraction by replacing the Ethanol by a mixing Ethanol-water and on the other hand by improving the techniques of microanalyses of the tolerance markers to wilt disease. The results showed that these modifications permitted to reach a quantity more important of phenol than the classic method. Besides, the analyses of the total phenol and the fongitoxicity permitted to discriminate the tolerant clones from the sensitive clones. These new methods allow us to evaluate faster the tolerance to wilt  disease of the clones after inoculation of the pathogenic agent Mots cles : Palmier a huile – Fusarium – Phenols – Clones – extraction.

sciences and nature. série a, biosciences agronomie environnement biotechnologie

Performance de deux techniques d’extraction des phénols racinaires pour l’évaluation du marquage de la tolérance à la fusariose des clones de palmier a huile (&lt;i&gt;Elaeis guineensis&lt;/i&gt; jacq.)