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The distribution of chlorocholine chloride (CCC) residue or its metabolites in the meat and eggs of laying hens was studied using the 15N delta value (d15N) and 15N atom % derived from 15N-CCC containing diets. In a completely randomised design, 20 laying hens were divided into four groups allocated four different diets namely; 0 mg 15N-CCC /kg feed a control diet (group A); 5 mg 15N-CCC /kg feed (group B), 50 mg 15N-CCC /kg (group C) and 100 mg 15N-CCC /kg (group D) for 11 days. During the seven days that followed, 15N-CCC diets were withdrawn and all hens were restored to feeding on the control diet. The d15N excess and 15N atom % excess in meat and eggs of hens fed diets containing 15N-CCC, were higher than in the control diet after 11 days of treatment and seven days after withdrawal of 15N-CCC, except for the egg yolk values of hens fed 5 mg 15N-CCC /kg feed. The d15N excess and 15N atom % excess of meat, egg yolk and egg albumen were dependent on dietary 15N-CCC concentrations and differed significantly between tissues for each of the three 15N-CCC concentrations examined. Femur meat d15N excess and 15N atom % excess were similar to that of breast meat but differed significantly from that of other tissues. The results show that tissue type is a factor in CCC residue / metabolite accumulation in chicken products suggesting differences in exposure or risk of CCC on consumers. Keywords: Chlorocholine chloride residues, Poultry products, Labeled-15N South African Journal of Animal Science Vol.33(4) 2003: 274-279

Chlorocholine chloride residue distribution in eggs, breast and femur meat of laying hens determined by labelled-<sup>15</sup>N estimates