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Anthropogenic activities are putting increasing pressure on ecosystems and raising the need to assess environmental  health quickly and accurately. Heterophile/lymphocyte index is accepted as a response to stress factors and the  micronucleus test is used as a biomarker to estimate genotoxic damage. To evaluate a model of environmental quality,  healthy Burrowing owl (Athene cunicularia) were sampled, and the heterophile/lymphocyte ratio was determined, as  well as the frequency of micronucleated erythrocytes and nuclear abnormalities, determined with the Wright-Giemsa  and acridine orange technique. The sites with the greatest antropic disturbance recorded the highest frequencies of  micronuclei and heterophile/lymphocyte index. The combination of both tests allows the detection of possible acute or  chronic exposure to stressors and genotoxic contaminants, both in healthy or altered ecosystems.

Anthropogenic Influence on Blood Biomarkers of Stress and Genotoxicity of the Burrowing Owl (Athene Cunicularia)