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This study was conducted to characterize the extracellular polymeric substances (EPS) produced by growing Cloacibacterium normanense in wastewater sludge alone and fortified crude glycerol. The EPS highest concentration of 17.5 g/L was produced using 25 g/L sludge suspended solids supplemented with 25 g/L of crude glycerol. Galactose and Glucose was the main compound of EPS produced with or without crude glycerol, respectively. EPS FTIR spectra revealed a variation in the different functional groups such as amines, carboxyl, and hydroxyl groups for EPS produced. Different functional groups were observed in the EPS produced with or without glycerol. The EPS exhibited kaolin flocculation activity up to 95% and was stable at high temperature. The high viscosity bioflocculant properties of EPS make it suitable for potential industrial applications

journal of material sciences and engineering

Fourier Transform Infrared Spectroscopy and Liquid Chromatography–Mass Spectrometry Study of Extracellular Polymer Substances Produced on Secondary Sludge Fortified with Crude Glycerol