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Brucellosis is a worldwide zoonosis with a high degree of morbidity in humans. It was formerly known as Mediterranean fever, Malta fever or undulant fever. The aim of this study to determine the prevalence of brucellosis among febrile negative malaria patients consume raw milk in West Darfur State, Sudan. In this cross sectional study, one hundred and half blood samples were collected from febrile patients and examined by Rose Bengal (RBPT), ELISA, SAT and PCR methods. The results showed 55 samples were positive by RBPT method, 67 samples were positive by SAT, 72 samples were positive by ELISA and 110 sample positive by nested PCR. Deficiency of awareness of brucellosis with prevailing routine habit of consumption raw milk and close contact with infected animals can function as mean of infection to human beings, molecular methods and could be a useful tool for the detection of Brucella spp.

Sero-Prevalence and Molecular Detection of Brucellosis among Febrile Patients in West Darfur State, Sudan