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Many papers have been published so far on gas plasma sterilization. They are mostly conducted by physical researchers, so microbiology and chemical aspects are significantly insufficient. By joining the biologists and chemists, gas plasma sterilization research was outstandingly advanced. The mechanism was not clear until now and from the life time and some other reasons metastables can be the most appropriate factors for sterilization. Spore death is explained from the hydration of dipicolinic acid in the core. By attacking metastables spore surface was pin hole opened and the interior water and surrounding water penetrate into the core to hydrate DPA. DPA in the surface was collected with water and enriched with SPE (solid phase extraction) column. SPE drain was analyzed with C-18 column and eluted with an acetonitrile/water (1/4, v/v) and detected with 235 nm. Spore surface particle can be confirmed DPA. The hydration process can cause within spore, so spore figures are unchanged before and after sterilization.

Role of Metastable and Spore Hydration to Sterilize Spores by Nitrogen Gas Plasma Exposure and DPA Analysis by HPLC and UV