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Acetobacter aceti was successfully used to induce transcriptional analysis, plant biochemical profile and nutritional contents in barley. Association of barley and microbial strain AC8 was reported best combination among other eight microbial strains of Bacillus in this study. AC8 microbial strain was identified as A. aceti from Fungal Culture Bank, Institute of Agricultural Science, University of the Punjab, Lahore, Pakistan. Different strains of A. aceti i.e., AC1, AC2, AC3… AC8 were analyzed as plant inducers and AC8 was screened out as best inducer in barley. It induced highest quantities of plant biochemical (i.e., phytosterols, phenolics, alkaloids and terpenoids) in barley. Current study revealed that among eight microbial strains AC8 had maximum potential to increase ascorbic acid, panthothenic acid, pyridoxine, thiamine and riboflavin in barley than other microbial strains. AC8 screened out among other microbial strains on the basis of its high vitamins induction potential. AC3 plus AC6 were reported second in the recorded list although other strains had a chronological reduction in vitamins as AC2>AC7>AC4>AC5 and AC1. Further evaluation of AC8 was done to check its efficiency for biochemicals, nutritional and isozyme contents induction in barley. Statistical analysis was performed using ANOVA and DMRT through DSAASTAT.

Evaluation of Bacterial Strains for the Induction of Plant Biochemicals, Nutritional Contents and Isozymes in Barley