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\udIn a CBRNe (Chemical, Biological, Radiological, Nuclear and explosive) scenario, biological agents hardly allow\udefficient detection/identification because of the incubation time that provides a lag in symptoms outbreak following\udtheir dissemination. The detection of atmospheric dispersion of biological agents (i.e.: toxins, viruses, bacteria and so\udon) is a key issue for the safety of people and security of environment. Another fundamental aspect is related to the\udefficiency of the sampling method, which leads to the identification of the agent released, in fact an effective sampling\udmethod is needed either to identify the contamination and to check for the decontamination procedure.\udEnvironmental monitoring is one of the ways to improve fast detection of biological agents; for instance, particle\udcounters with the ability of discriminating between biological and non-biological particles are used for a first warning\udwhen the amount of biological particles exceeds a particular threshold. Nevertheless, these systems are not able to\uddistinguish between pathogen and non-pathogen organisms, thus, classical “laboratory” assays are still required to\udunambiguously identify the particle which triggered the warning signal. In this work, a combination of commercially\udavailable equipment for detection and identification of the atmospheric dispersion of biological agents was evaluated in\udpartnership between the Italian Army, the Department of Industrial Engineering and the School of Medicine and\udSurgery of the University of Rome “Tor Vergata”. The aim of this work, whose results are presented here, was to\udconduce preliminary studies on the dynamics of biological aerosols fallout after its dispersion, to improve detection,\udsampling and identification techniques. This will help minimizing the impact of the release of biological agents,\udguarantee environmental, and people safety and securit

journal of microbial and biochemical technology

Use of Particle Counter System for the Optimization of Sampling, Identification and Decontamination Procedures for Biological Aerosols Dispersion in Confined Environment