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The objective of this study was to evaluate the effects of growth hormone (GH) and muscle strength training (ST) on the composition of bone tissue of Wistar rats through Raman spectroscopy. In total, 40 male rats were randomly distributed i nto fo r groups: (N = 10) control (C), control with the application of GH (GHC), strength training (T), and strength training with the a pplication of GH (GHT). The training consisted of four series of 10 water jumps, performed three times a week, with an overload correspond ing to 50 % of body weight and duration of four weeks. GH was applied at a dose of 0.2 IU / kg in each animal three times a week and e very other day. After four weeks, the animals were euthanized and the right femurs collected for analysis of the bone structure. Ram an spectroscopy (ER) was used to observe the following compounds from their respective bands: Calcium Carbonate-Triglycerides (fat ty acids) 1073 cm, Collagen type I 509 cm , Bone-DNA Phosphate (Protein) 589 cm , Phosphate Phospholipids 1078 cm . For the statistical analysis, the Shapiro-Wilk and ANOVA One-Way variance analysis normality tests were performed, followed by the Tuke y post-test. The results showed an increase in the concentrations of calcium carbonate-triglycerides (fatty acids), type I collag en, bone phosphate-DNA (protein), and phosphate phospholipids in all experimental groups, with or without ST and/or GH , But only the is lated training group differed significantly from the control group (P <0.05). It was concluded that all treatments could promote bone tissue gain, however, only the T group demonstrated a significant difference in the mineral compounds analyzed.

Muscle Strength Training is Better than the Use of Growth Hormone (GH) in Bone Health of Wistar Rats