Open AccessCD36 Is Differentially Expressed by CD8+ Splenic Dendritic Cells But Is Not Required for Cross-Presentation In Vivo
Author(s) -
Gabrielle T. Belz,
David Vremec,
Maria Febbraio,
Lynn M. Corcoran,
Ken Shortman,
Federico Carbone,
William R. Heath
Publication year - 2002
Publication title -
the journal of immunology/the journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.737
H-Index - 372
eISSN - 1550-6606
pISSN - 0022-1767
DOI - 10.4049/jimmunol.168.12.6066
Subject(s) - cross presentation , antigen presentation , cytotoxic t cell , cd8 , microbiology and biotechnology , cd36 , biology , priming (agriculture) , dendritic cell , in vivo , spleen , mhc class i , in vitro , immunology , immune system , receptor , genetics , germination , botany
Cross-presentation allows the processing of Ags from donor cells into the MHC class I presentation pathway of dendritic cells (DCs). This is important for the generation of cytotoxic T cell immunity and for induction of self tolerance. Apoptotic cells are reported to be efficient targets for cross-presentation, and in vitro studies using human DCs have implicated CD36 in their capture. In support of a role for CD36 in cross-presentation, we show that this molecule is differentially expressed by CD8(+) splenic DCs, which previously have been identified as responsible for cross-presentation in the mouse. Three different cross-presentation models were examined for their dependence on CD36. These included cross-priming to OVA-coated spleen cells and cross-tolerance to OVA transgenically expressed in the pancreatic islet beta cells under constitutive conditions or during beta cell destruction. In these models, CD36 knockout DCs were equivalent to wild-type DCs in their capacity to cross-present either foreign or self Ags, indicating that CD36 is not essential for cross-presentation of cellular Ags in vivo.