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The study of the hydrodynamic behavior in reactors provides characteristics of the flow regime and its anomalies that can reduce biological processes efficiency due to the decrease of the useful volume and the hydraulic retention time required for the performance of microbial activity. In this study, the hydrodynamic behavior of an anaerobic-aerobic fixed bed reactor, operated with HRT (hydraulic retention time) of 24, 18 and 12 hours, was evaluated in the treatment of raw cattle slaughterhouse wastewater. Polyurethane foam and expanded clay were used as support media for biomass immobilization. Experimental data of pulse type stimulus-response assays were performed with eosin Y and bromophenol blue, and adjusted to the single-parameter theoretical models of dispersion and N-continuous stirred tank reactors in series (N-CSTR). N-CSTR model presented the best adjustment for the HRT and tracers evaluated. RDT (residence time distribution) curves obtained with N-CSTR model in the assays with bromophenol blue resulted in better adjustment compared to the eosin Y. The predominant flow regime in AAFBR (anaerobic aerobic fixed bed reactor) is the N-CSTR in series, as well as the existence of preferential paths and hydraulic short-circuiting

<b>Effect of hydraulic retention time on hydrodynamic behavior of anaerobic-aerobic fixed bed reactor treating cattle slaughterhouse effluent