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Eight lipid patterns of Silybum marianum (L.) Gaertn seed oil, not hitherto reported, (20%) have been elucidated in this laboratory using capillary GLC and HPLC as main tools of analysis. The oil is rich in linoleic acid (53.3%) and oleic acid (21.3%). Five major triacylglycerols containing linoleic acyls namely LLL, LLO, LLP, LOO and LOP were detected by HPLC using FID detector, these triacylglycerols are also predominating in both cottonseed and sunflower oils but in different proportions. The total tocopherols content (260 ppm) was determined directly in the oil by HPLC. It was found that the oil contains alpha -tocopherol as a major constituent (84.5%) resembling sunflower oil. The whole sterols pattern, as determined as their trimethylsilyl derivatives by GLC, includes campesterol, 5-stigmasterol, beta-sitosterol, 7-stigmasterol, avenasterol and spinasterol. The four sterols lipid clases of free and acylated sterols and sterylglycosides have been determined as their 9- and 1- anthroylnitrile derivatives by fluorescence and UV- HPLC respectively.

Detailed studies on some lipids of <i>Silybum marianum</i>(L.) seed oil