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Leukotrienes have been implicated as important mediator in a bronchial asthma. The purpose of this study is to evaluate the degree of participation of leukotrienes on onset of symptoms of nasal allergy. We measured leukotriene C4 and D4 (LTC4 and LTD4) in nasal secretion by radioimmunoassay combined with high performance liquid chromatography. This method is superior to the one previously reported in specificity, sensitivity and reproducibility. Subjects consisted of 10 patients (aged 8-34 years, average 19) with house dust nasal allergy. The nasal challenge was performed by applying antigen discs on bilateral inferior turbinates for 10 minutes. During the 10 minutes challenge with antigen, the total amount of nasal secretion was collected by means of suction

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Nasal allergy and leukotriene. 1. Extraction and measurement of LTC4 and LTD4 in nasal secretion induced by antigen challenge, and the effect of LTC4 and LTD4 on nasal mucosa.