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Multiple-locus variable number of tandem repeats analysis (MLVA) is useful for epidemiological investigations. In this study, MLVA-18 was used to assess the genetic and epidemiological relationships of Brucella canis isolates among a domestic dog and dogs from two breeding farms. MLVA-18 assay showed variation in copy numbers in 6 of the 18 loci. Interestingly, the YJ02-1 strain isolated from the pet dog from Yangju-si showed only one tandem repeat change in Bruce 09 locus as compared with that of NYJ-1 strain isolated from a dog from the Namyangju-si farm. The two strains were shown to have originated from the same source or a closely related strain.

Multiple-locus variable number of tandem repeat analysis assay as a tool for the epidemiological study of Brucella canis in Korea