English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The larval stages of the central European sibling caddisfly species    Sericostoma   personatum    (Spence in Kirby and Spence, 1826) and    S.   flavicorne    Schneider, 1845 are morphologically similar and can only be distinguished by differences in coloration in late larval instars. Identification using the mitochondrial barcoding gene, i.e., the Cytochrome c Oxidase 1, is impossible, as both species share the same highly differentiated haplotypes due to introgression. Nuclear gene markers obtained through double digest restriction site associate sequencing (ddRAD seq), however, can reliably distinguish both species, yet the method is expensive as well as time-consuming and therefore not practicable for species determination. To facilitate accurate species identification without sequencing genome-wide markers, we developed nine diagnostic nuclear  RFLP  markers based on ddRAD seq data. The markers were successfully tested on geographically distinct populations of the two    Sericostoma    species in western Germany, on known hybrids, and on another sericostomatid caddisfly species,    Oecismus   monedula    (Hagen, 1859) that sometimes shares the habitat and can be morphologically confounded with    Sericostoma   . We describe a simple and fast protocol for reliable species identification of    S.   personatum    and    S.   flavicorne    independent of the life cycle stage of the specimens.

zookeys

When barcoding fails: development of diagnostic nuclear markers for the sibling caddisfly species    Sericostoma   personatum    (Spence in Kirby &amp; Spence, 1826) and    Sericostoma   flavicorne    Schneider, 1845