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A new species of frog of the genus    Pristimantis    is described from a montane forest between 1700 and 1800 m a.s.l. of the Pui Pui Protected Forest (Región Junín) in central Peru.    Pristimantis   ashaninka    sp. n.  is described based on five adult females (snout–vent length 23.1–26.7 mm) and ten juveniles (snout-vent length 10.6–13.4). It differs from its congeners by having the skin on dorsum shagreen with many conical tubercles giving it a spinose appearance, lacking a tympanum, having groin, anterior and posterior surfaces of thighs uniformly grayish brown, and a pale bronze iris with fine black reticulations, a median reddish hint horizontally across iris, and a black narrow vertical streak from pupil across lower and upper half of iris. Among the Peruvian    Pristimantis    that lack a tympanum,    P.   ashaninka    sp. n.  is morphologically most similar to    P.   lirellus   ,    P.   martiae   , and    P.   rhabdocnemus   . However, 16S DNA barcoding revealed clear distinctions between all four species of    Pristimantis   .

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A new species of    Pristimantis    (  Amphibia  ,   Anura  ,   Craugastoridae  ) from a montane forest of the Pui Pui Protected Forest in central Peru (Región Junín)