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Purslane (   Portulaca   oleracea    L.,   Portulacaceae  ) is a widespread edible plant with significant ethnobotanical and ethnopharmacological importance. The plant is characteristic for the presence of a class of indoline amide glucoside alkaloids, called cyclo-dopa amides, or oleraceins. Additionally, a new, structurally similar to oleraceins, class of indole amides have been discovered recently, called oleraindoles. These compounds have been evaluated to possess antiinflammatory and anticholinesterase activities. Herein, utilizing  UHPLC -Orbitrap-MS with MS 2  filtering by diagnostic ion filtering ( DIF ), and diagnostic difference filtering ( DDF ) using different data analysis tools, eight compounds with oleraindole structure were tentatively identified.

﻿ UHPLC -Orbitrap screening of oleraindoles in hydromethanolic extracts of Portulaca oleracea

UHPLC -Orbitrap screening of oleraindoles in hydromethanolic extracts of Portulaca oleracea