Open AccessEstablishment and assessment of two methods for quantitative detection of serum duck hepatitis B virus DNA
Author(s) -
Yaxi Chen,
Aiping Huang,
Qi Zhang,
Shuai Guo
Publication year - 2004
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v10.i18.2666
Subject(s) - duck hepatitis b virus , digoxigenin , microbiology and biotechnology , primer (cosmetics) , dna , biology , southern blot , hybridization probe , alkaline phosphatase , dot blot , chemistry , gene , virology , hepatitis b virus , virus , hepadnaviridae , biochemistry , in situ hybridization , gene expression , enzyme , organic chemistry
To establish and assess the methods for quantitative detection of serum duck hepatitis B virus (DHBV) DNA by quantitative membrane hybridization using DHBV DNA probe labeled directly with alkaline phosphatase and fluorescence quantitative PCR (qPCR).