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The current study was aimed to observe the prevalence of Helicobacter pylori infection at District Mardan with respect to their gender and age groups. The blood samples were collected from all the subjects who attended the OPD at Mardan Medical Complex. All these samples were screened for the presence of antibodies against H. pylori through a rapid antibody assay. Positive and negative cases were recorded based on the test results and prevalence was measured keeping in mind the selected parameters in individuals of each group. A total of 4312 people was included in the study, out of which 2586 were males and 1726 were females. Majority of the patients were in the mature age group. Based on our results, there were 125 males demonstrating a prevalence of 4.83% which is slightly higher than 4.66% prevalence of 77 females infected. Comparisons were made based on gender and age groups and data presented in the form of figures and tables. Monthly wise prevalence has also been shown. Moreover, prevalence was higher in the people aged 19-44 and lowest in immature ones. However, the overall prevalence in the subjects was 4.68% for the span of 4 months.