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Original Research Article Background: Cervical cancer (CC) is a major public health problem worldwide. In Senegal, it is the first female cancer before breast cancer. Human papillomavirus (HPV) has been described as responsible for CC and is the most common sexually transmitted infection in the world. Low risk HPV (HPV-LR) including HPV 6 and HPV 11 are responsible for most of mild infections while among high-risk HPV (HPV-HR) including genotypes 16, 18, 45, 31, 33, 52, 58, 35 in order of frequency are involved in almost 90% of cervical cancer. Objective: The general objective is to do molecular genotyping of HPVs and to assess the prevalence of HPV types among Senegalese women. Materials and methods: The study was done on a population of 198 sexually active women with normal cytology. DNA extraction is carried out using endocervical samples stored in Abbott Cervi-Collect Specimen Collection Kit tubes with the Zymo Research DNA Viral KIT TM , USA www.Zymoresearch.com. Molecular identification of the HPV genotypes (19 HPV-HR and 9 HPV-LR) was carried out by multiplex PCR with the Anyplex TM II HPV28 Detection kit from Seegene on a CFX96TM Bio-Rad machine. Data analysis was performed by R Studio software (3.6.0), Pearson chi-square comparisons or Fisher's exact test. Odds Ratios were calculated to estimate the association between individual characteristics and HPV infection if P<5%. Results: 64.65% (128/198) Women were infected with HPV. HPV-HR and HPV-LR had respective prevalences of 57.07% (113/198) and 34.34% (68/128). 37.37% of the women presented a co-infection, that is to say hosting at least two genotypes. The most common genotypes were HPV56 (27.78%), HPV68 (12.12%), HPV52 (8.59%), HPV31 (8.08%), HPV58 and HPV82 (7.07% each) in HPV-HR and among HPV-LR HPV 42 (14.14%), HPV54 (8.08%), HPV43 and HPV44 (5.05%, each). HPV16 and HPV18 had a prevalence of 7.07% and 5.05% respectively and HPV-6 and HPV-11 also had a prevalence of 4.54% and 0.51% respectively. Our results showed that there was an association between HPV infection, age, marital status and contraception. Conclusion: Our results show high prevalence of HPV HPV-HR and HPV-LR in our population of women with normal cytology. The number of co-infected women who harbored at least two genotypes was high. The non-vaccine genotypes are mainly identified (HPV56, HPV42, HPV68, HPV52, HPV31, HPV54 et HPV82). The HPV16 and HPV18 genotypes were present at a moderate level. The woman's marital status, the woman’s age and contraception were associated with HPV infection. These results contribute to a better understanding of the molecular epidemiology of HPV in Senegal.

Molecular Genotyping of Human Papilloma Virus (HPV) in Women with Normal Endo-Cervical Cytology in Senegal