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Dental caries is one of the worldwide common disease affecting both rural and urban communities. Demineralization is the loss of significant amount of minerals, especially calcium and phosphate from the tooth enamel leading to caries formation. The process of demineralization and remineralization is considered as a dynamic process, characterized by the flow of phosphate and calcium out of and back in the enamel. Therefore, the current in-vitro study was designed for evaluating the remineralization potential of casein phospho-peptide with amorphous calcium phosphate fluoride (CPP-ACPF) on the artificially demineralized enamel surfaces samples. Thirty enamel samples were subjected to artificial demineralization procedure followed by remineralization using CPP-ACPF for one week in ten samples, two weeks in next ten sample and four-week s in last ten samples. The final results were analysed using the Confocal Laser Scanning Microscopic (CLSM) and Image J software. The mean score of remineralization potential for one, two- and four-week samples was found to be 59.1064, 58.96 and 56.95 respectively. The mean gray value scores were almost similar in one, two- and four-week samples i.e., the remineralization potential does not increase or decrease significantly with increasing time. But the overall CPP-ACPF remineralization potential was high and therefore it can be used as a potential agent to prevent dental caries. The data from our current study will help to make clinical use of the agent as a potential and excellent local slow-delivery system to treat the white spot lesion in enamel.

biomedical research-tokyo

Evaluation of remineralization potential of casein phospho-peptide with amorphous calcium phosphate fluoride (CPP-ACPF) on demineralised enamel surfaces.