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There is an increasing demand for bone graft substitutes that are used as osteoconductive scaffolds in the treatment of bone defects and fractures. Achieving optimal bone regeneration requires initial cell seeding of the scaffolds prior to implantation.  In order to achieve an efficient seeding of the scaffolds, a novel cell seeding chamber was developed. The cell seeding chamber is a closed assembly that works like an hourglass. The position of the scaffold is between two reservoirs containing the cell suspension  (e.g., blood or autologous bone marrow). The cell suspension at the upper reservoir flows through the scaffold by gravitational force. The cell suspension is collected at the lower reservoir. When the upper reservoir is empty the whole assembly is turned and the process starts again. In this study, a new compact cell seeding chamber for initial cell seeding has been developed that can be used in situ. The basic functionality of the cell seeding chamber was demonstrated with a blood substitute

A Novel Cell Seeding Chamber for Tissue Engineering and Regenerative Medicine