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Background:  Leishmaniasis is a zoonotic disease caused by protozoan parasites from  Leishmania  genus. Currently, there are no effective vaccines available and the available therapies are far from ideal. In particular, the development of new therapeutic strategies to reduce the infection caused by  Leishmania amazonensis  could be considered desirable. Different plant-derived products have demonstrated antileishmanial activity, including the essential oil (EO) from  Artemisia absinthium  L. (EO-Aa), Asteraceae.  Methods:  In the present study, the EO-Aa formulated in nanocochleates (EO-Aa-NC) was investigated in vitro against intracellular amastigotes of  L. amazonensis  and non-infected macrophages from BALB/c mice. In addition, the EO-Aa-NC was also evaluated in vivo against on experimental cutaneous leishmaniasis, which body weight, lesion progression, and parasite load were determined.  Results:  EO-Aa-NC displayed IC 50  values of 21.5 ± 2.5 μg/mL and 27.7 ± 5.6 μg/mL against intracellular amastigotes of  L. amazonensis  and non-infected peritoneal macrophage, respectively. In the animal model, the EO-Aa-NC (30 mg/kg/intralesional route/every 4 days 4 times) showed no deaths or weight loss greater than 10%. In parallel, the EO-Aa-NC suppressed the infection in the murine model by approximately 50%, which was statistically superior ( p  < 0.05) than controls and mice treated with EO-Aa. In comparison with Glucantime ® , EO-Aa-NC inhibited the progression of infection as efficiently ( p  > 0.05) as administration of the reference drug.  Conclusions:  Encochleation of EO-Aa resulted in a stable, tolerable, and efficacious antileishmanial formulation, facilitating systemic delivery of EO, with increased activity compared to administration of the free EO-Aa. This new formulation shows promising potential to future studies aimed at a new therapeutic strategy to treat leishmaniasis.

In Vitro and In Vivo Evaluation of Essential Oil from Artemisia absinthium L. Formulated in Nanocochleates against Cutaneous Leishmaniasis