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Three new secondary metabolites, actinoflavosides B-D ( 1 - 3 ), were discovered in the culture broth of two actinomycete strains (JML48 and JMS33) that were isolated from tidal mudflat sediment in Muan, Republic of Korea. The planar structures of the actinoflavosides were elucidated by MS, UV, and NMR analyses. The stereochemistry of an aminosugar, 2,3,6-trideoxy-3-amino-ribopyranoside in the actinoflavosides was determined by J -based configuration analysis using values obtained from DQF-COSY experiments and modified Mosher's method. Actinoflavosides B-D ( 1 - 3 ) displayed antibacterial activity against Pseudomonas aeruginosa , and actinoflavoside D ( 3 ) significantly increased IL-2 production in mouse splenocytes.

Actinoflavosides B–D, Flavonoid Type Glycosides from Tidal Mudflat-Derived Actinomyces