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Corynebacterium glutamicum  is widely used for amino acid production. In the present study, 543 genes showed a significant change in their mRNA expression levels in  l -lysine-producing  C .  glutamicum  ATCC21300 than that in the wild-type  C .  glutamicum  ATCC13032. Among these 543 differentially expressed genes (DEGs), 28 genes were up- or downregulated. In addition, 454 DEGs were functionally enriched and categorized based on BLAST sequence homologies and gene ontology (GO) annotations using the Blast2GO software. Interestingly, NCgl0071 ( bioB , encoding biotin synthase) was expressed at levels ~20-fold higher in the  l -lysine-producing ATCC21300 strain than that in the wild-type ATCC13032 strain. Five other genes involved in biotin metabolism or transport—NCgl2515 ( bioA , encoding adenosylmethionine-8-amino-7-oxononanoate aminotransferase), NCgl2516 ( bioD , encoding dithiobiotin synthetase), NCgl1883, NCgl1884, and NCgl1885—were also expressed at significantly higher levels in the  l -lysine-producing ATCC21300 strain than that in the wild-type ATCC13032 strain, which we determined using both next-generation RNA sequencing and quantitative real-time PCR analysis. When we disrupted the  bioB  gene in  C .  glutamicum  ATCC21300,  l -lysine production decreased by approximately 76%, and the three genes involved in biotin transport (NCgl1883, NCgl1884, and NCgl1885) were significantly downregulated. These results will be helpful to improve our understanding of  C .  glutamicum  for industrial amino acid production.

international journal of molecular sciences

Transcriptome and Gene Ontology (GO) Enrichment Analysis Reveals Genes Involved in Biotin Metabolism That Affect l-Lysine Production in Corynebacterium glutamicum