English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

(1) Background: It is known that RNA in Na+ salt form has only immunomodulatory activity, and in acid form additionally acquires anti-inflammatory activity and in combination with D-mannitol acquires an even broader antiviral effect. The study aimed to study the ability of adenosinmonophosphat (AMP) and oligoribonucleotides (ORNs) in acid and salt form and in combination with D-mannitol to affect the conformation and fluorescence of interferon (INF) α-2b and to determine the energy parameters of these interactions. (2) Methods: spectroscopy (time pulsed and fluorescence), isothermal nanocalorimetry. (3) Results: AMP and ORNs in acid form and complex with D-mannitol bind more strongly to interferon α-2b than salt analogues. In the interaction of interferon α-2b and acid AMP and especially in complex with D-mannitol, the reaction occurs exothermically and change in conformational mobility INF by increasing the content of disordered regions. When INF α-2b interacts with salt AMP, the reaction occurs endothermically, and probably the salt form increases the conformational stiffness of INF α-2b. The greater efficiency of nonradiative energy transfer from INF α-2b to acid AMP has been shown, due to the closer distance between molecules. (4) Conclusions: AMP in acid form interacts more actively and increases the conformational mobility of INF, at a greater relative distance and with less Gibbs energy compared to the salt form, which probably causes the appearance of additional biological properties of acidic AMP.

Conformational, Fluorescence and Energy Parameters of Interferon α2b with Different Forms of Oligoribonucleotides and Adenosine Monophosphate