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A laser technology for creating nanocomposites from alternating layers of albumin/collagen proteins with two types of single-walled carbon nanotubes (SWCNT) at concentrations of 0.001 and 0.01 wt.% was proposed. For this purpose, a setup with a diode laser (810 nm) and feedback system for controlling the temperature of the area affected by the radiation was developed. Raman spectroscopy was used to determine a decrease in the defectiveness of SWCNT with an increase in their concentration in the nanocomposite due to the formation of branched 3D networks with covalent bonds between nanotubes. It was revealed that adhesion of proteins to branched 3D networks from SWCNT occurred. The specific electrical conductivity of nanocomposites based on large SWCNT nanotubes was 3.2 and 4.3 S/m compared to that for nanocomposites based on small SWCNT with the same concentrations—1.1 and 1.8 S/m. An increase in the concentration and size of nanotubes provides higher porosity of nanocomposites. For small SWCNT-based nanocomposites, a significant number of mesopores up to 50 nm in size and the largest specific surface area and specific pore volume were found. Nanocomposites with small SWCNT (0.001 wt.%) provided the best cardiac fibroblast viability. Such technology can be potentially used to create bioelectronic components or scaffolds for heart tissue engineering.

applied sciences

Laser Technology for the Formation of Bioelectronic Nanocomposites Based on Single-Walled Carbon Nanotubes and Proteins with Different Structures, Electrical Conductivity and Biocompatibility