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Proliferative enteropathy (PE) is an infectious enteric disease caused by  Lawsonia intracellularis  ( L. intracellularis ) and is endemic in pig herds worldwide. However, a  L. intracellularis -specific monoclonal antibody plays an important role in the evaluation of  L. intracellularis  infection  in vitro . Therefore, the objective of this study was to produce and identify the characteristics of a new monoclonal antibody against the outer membrane protein (Omp2) of  L. intracellularis  and apply it in an indirect immunofluorescence assay (IFA) and immunocytochemistry (IHC). The results indicated that three highly specific monoclonal antibodies against the Omp2 protein (4D9, 3G2, and 7G5) of  L. intracellularis  were obtained by using purified Omp2 as an immunogen, the titers of ascitic fluids of 4D9, 3G2, and 7G5 cells were 1:2,048,000, 1:512,000, and 1:256,000, respectively. IFA analysis showed that the 4D9, 3G2, and 7G5 have no cross-reactivity with other enteric bacteria commonly found in the ilea of pigs or closely related to  L. intracellularis , such as  Desulfovibrio, Bilophila wadsworthia  ( B. wadsworthia ),  Salmonella choleraesuis  ( S. choleraesuis ),  Salmonella typhimurium  ( S. typhimurium ),  Escherichia coli  ( E. coli ), and  Brachyspira hyodysenteriae  ( B. hyodysenteriae ). IFA and IHC results indicated that the monoclonal antibodies can be successfully used as primary antibodies to detect  L. intracellularis  in infected cells and in the crypt of the ileum from infected tissues of PE. Our findings suggested that the new monoclonal antibody specific against  L. intracellularis  will be useful for the evaluation of  L. intracellularis  infection  in vivo  and  in vitro .

frontiers in veterinary science

Preparation and Characterization of a New Monoclonal Antibody Specific Against Lawsonia intracellularis and Its Application in Indirect Immunofluorescence and Immunocytochemistry Assay