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Clubroot caused by  Plasmodiophora brassicae  is a severe threat to the production of  Brassica napus , worldwide. The cultivation of resistant varieties is the most efficient and environmentally friendly way to limit disease spread. We developed a highly resistant  B. napus  line, ZHE226, containing the resistance locus  PbBa8.1 . However, ZHE226 seeds contain high erucic acid content, which limits its cultivation owing to its low edible oil quality. A segregation population of BC 3 F 2  was developed by crossing ECD04, a resistant European turnip donor, with Huangshuang5, an elite variety with no erucic acid in its seeds, as a recurrent plant. Fine mapping using the bulk segregation analysis sequencing (BSA-Seq) approach detected  PbBa8.1  within a 2.9 MB region on chromosome A08. Interestingly, the previously reported resistance gene  Crr1a  was found in the same region. Genetic analysis revealed that the CAP-134 marker for  Crr1a  was closely linked with clubroot resistance (CR). Thus,  PbBa8.1  and  Crr1a  might be allelic for CR. Moreover, comparative and genetic analysis showed that high erucic acid in the seeds of ZHE226 was due to linkage drag of  fatty acid elongase 1  ( FAE1 ) in the ECD04 line, which was located in the interval of  PbBa8.1  with a physical and genetic distance of 729 Kb and 1.86 cm, respectively. Finally, a clubroot-resistant line with a low erucic acid content was successfully developed through gene-specific molecular marker assistant selection from BC 4 F 4 . These results will accelerate CR breeding programs in  B. napus.

Association of Clubroot Resistance Locus PbBa8.1 With a Linkage Drag of High Erucic Acid Content in the Seed of the European Turnip