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Nanocomposite materials are multi-phase materials, usually solids, which have two or more component materials having different chemical and physical properties. When blended together, a newer material is formed with distinctive properties which make them an eligible candidate for many important applications. In the present study, thin films of nafion (polymer) and hematite or α-Fe 2 O 3  (nanoparticles) nanocomposite is fabricated on indium tin oxide (ITO) coated glass substrates, due to its enhanced ionic conductivity, for cholesterol biosensor applications. Scanning electron microscopy and Atomic force microscopy revealed the formation of nanorod structured α-Fe 2 O 3  in the films. The cyclic voltammetry (CV) studies of nafion-α-Fe 2 O 3 /ITO revealed the redox properties of the nanocomposites. The sensing studies were performed on nafion-α-Fe 2 O 3 /CHOx/ITO bioelectrode using differential pulse voltammetry (DPV) at various concentrations of cholesterol. The enzyme immobilization leaded to the selective detection of cholesterol with a sensitivity of 64.93 × 10 −2  μA (mg/dl) −1  cm −2 . The enzyme substrate interaction (Michaelis–Menten) constant K m , was obtained to be 19 mg/dl.

Investigating the Nanocomposite Thin Films of Hematite α-Fe2O3 and Nafion for Cholesterol Biosensing Applications