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A novel actinobacterium, strain YIM 96748 T , was isolated from a saline soil sample collected from the south bank of Aiding Lake in Xinjiang Province, Northwest China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 96748 T  is closely related to  Amycolatopsis cihanbeyliensis  BNT52 T  (98.9%) and  Amycolatopsis jiangsuensis  KLBMP 1262 T  (97.2%). The DNA–DNA relatedness between strain YIM 96748 T  and its closest type strain  A. cihanbeyliensis  BNT52 T  was 59.6%. The average nucleotide identity between strain YIM 96748 T  and its neighbor strain was 88.97%. Based on the genotypic and phenotypic characteristics, it is concluded that strain YIM 96748 T  represents a novel species of the genus  Amycolatopsis , whose name was proposed as  Amycolatopsis aidingensis  sp. nov. The type strain is YIM 96748 T . To investigate the biosynthetic potential of producing secondary metabolites, the complete genome of YIM 96748 T  was sequenced and analyzed. The complete genome sequence of YIM 96748 T  consists of a 7,657,695-bp circular chromosome, comprising 7,162 predicted genes with a DNA G + C content of 70.21 mol%. Fifty-one putative biosynthetic gene clusters of secondary metabolites were found, including the antibacterial/antitumor agent TLN-05220, the antibacterial agent nocardicin A, the antifungal agent nystatin A1, and the osmolyte ectoine. The investigation of the secondary metabolites of  A. aidingensis  YIM96748 T  led to the discovery of two new phenylpropyl acetate enantiomers, amycoletates A (1) and B (2), and five known compounds: 4-hydroxy phenethyl acetate (3), 2- p -acetoxyphenylethanol (4), ( S )-ethyl indole-3-lactate (5), ( R )-ethyl indole-3-lactate (6), and  p -hydroxybenzoic acid (7). One of the gene clusters 14, 36, and 43, which contain a single module of polyketide synthase, might be responsible for the biosynthesis of compounds 1 and 2 from compound 7 as a precursor. Further studies, including the one strain many compounds approach (OSMAC) and genetic modification, are needed to explore novel compounds from this talented halophilic  Amycolatopsis  strain.

Amycolatopsis aidingensis sp. nov., a Halotolerant Actinobacterium, Produces New Secondary Metabolites