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Berberine hydrochloride is an isoquinoline alkaloid, which has antitumoral, antibacterial, and antiviral activities  in vivo  and  in vitro .  Charybdis japonica  is one of the main economic species of crab in Southeast Asia. We studied the molecular mechanism of oxidative stress in berberine hydrochloride-treated  C. japonica  infected with  Aeromonas hydrophila .  C. japonica  were infected with  A. hydrophila  after being submerged in different concentrations (0, 100, 200, and 300 mg/L) of berberine hydrochloride for 48 h. The full-length cDNA of  Prx6  and the ORFs of  Prx5  and PXL2A were cloned.  Prx6  and  PXL2A  each have one conserved domain,  Cys44 , and  Cys81 . The  Prx5  conserved domain contains three important  Cys  loci,  Cys75 ,  Cys100 , and  Cys76 .  Prx6  was different from  Prx5  and  PXL2A  in the Peroxiredoxin family. The transcription levels of  PXL2A  infected with  A. hydrophila  were all higher than the control. The transcription levels of  C. japonica  were further increased by adding berberine hydrochloride and were increased the highest at a concentration of 300 mg/L. The activities of glutathione peroxidase, superoxide dismutase, and catalase in the hepatopancreas of berberine hydrochloride-treated  C. japonica  infected with  A. hydrophila  were significantly increased compared with those only infected with  A. hydrophila  and the control group. The glutathione transferase activity in the hepatopancreas was significantly increased in berberine hydrochloride-treated  C. japonica . The results of this study provide a new understanding of the potential role of berberine hydrochloride on the oxidative stress mechanisms of  C. japonica .

frontiers in marine science

Molecular Characterization of Prdx Family and Response of Antioxidant Enzymes in Berberine Hydrochloride-Treated Charybdis japonica Infected With Aeromonas hydrophila