English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

P48/45 is a conserved gametocyte antigen involved in  Plasmodium  parasite fertilization. A recombinant  Plasmodium vivax  P48/45 ( Pvs 48/45) protein expressed in  Escherichia coli  ( E. coli ) was highly antigenic and immunogenic in experimental animals and elicited specific transmission-blocking (TB) antibodies in a previous pilot study. Here, a similar  Pvs 48/45 gene was expressed in Chinese Hamster Ovary (CHO) cells and we compared its immunoreactivity with the  E. coli  product. Specific antibody titers were determined using plasma from Colombian individuals (n=227) living in endemic areas where both  P. vivax  and  P. falciparum  are prevalent and from Guatemala (n=54) where  P. vivax  is highly prevalent. In Colombia, plasma seroprevalence to CHO- rPvs 48/45 protein was 46.3%, while for  E. coli - rPvs 48/45 protein was 36.1% ( p< 0.001). In Guatemala, the sero prevalence was 24.1% and 14.8% ( p< 0.001), respectively. Reactivity index (RI) against both proteins showed an age-dependent increase. IgG2 was the predominant subclass and the antibody avidity index evaluated by ELISA ranged between 4-6 mol/L.  Ex vivo P. vivax  mosquito direct membrane feeding assays (DMFA) performed in presence of study plasmas, displayed significant parasite transmission-blocking (TB), however, there was no direct correlation between antibody titers and oocysts transmission reduction activity (%TRA). Nevertheless, DMFA with CHO  rPvs 48/45 affinity purified IgG showed a dose response; 90.2% TRA at 100 μg/mL and 71.8% inhibition at 10 μg/mL. In conclusion, the CHO-r Pvs 48/45 protein was more immunoreactive in most of the malaria endemic places studied, and CHO-r Pvs 48/45 specific IgG showed functional activity, supporting further testing of the protein vaccine potential.

frontiers in immunology

Immunoreactivity of Sera From Low to Moderate Malaria-Endemic Areas Against Plasmodium vivax rPvs48/45 Proteins Produced in Escherichia coli and Chinese Hamster Ovary Systems