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Molting and ovulation are the basic processes responsible for the growth and reproduction of  Macrobrachium nipponense ; however, the molecular mechanisms of molting and ovulation in  M. nipponense  are poorly understood. The present study aimed to use  MnFtz-f1  as the starting point to study the molting and ovulation phenomena in  M. nipponense  at the molecular level. The full-length  MnFtz-f1  cDNA sequence was 2,198 base pairs (bp) in length with an open reading frame of 1,899 bp encoding 632 amino acids. Quantitative real-time PCR analysis showed that  MnFtz-f1  was highly expressed in the ovary at the cleavage stage and on the fifth day after hatching.  In vivo  administration of 20-hydroxyecdysone (20E) showed that 20E effectively inhibited the expression of the  MnFtz-f1  gene, and the silencing of the  MnFtz-f1  gene reduced the content of 20E in the ovary.  In situ  hybridization (ISH) analysis revealed the localization of  MnFtz-f1  in the ovary. Silencing of  MnFtz-f1  by RNA interference (RNAi) resulted in significant inhibition of the expression of the vitellogenin ( Vg ),  Spook , and  Phantom  genes, thus confirming that  MnFtz-f1  had a mutual regulatory relationship with  Vg ,  Spook , and  Phantom . After RNAi, the molting frequency and ovulation number of  M. nipponense  decreased significantly, which demonstrated that  MnFtz-f1  played a pivotal role in the process of molting and ovulation.

MnFtz-f1 Is Required for Molting and Ovulation of the Oriental River Prawn Macrobrachium nipponense