English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Pyruvate dehydrogenase E1 ( PDHE1 ) is thought to play essential roles in energy metabolism, and a previous study suggested that it also has potential regulatory roles in male sexual development in the oriental river prawn,  Macrobrachium nipponense . In this study, we used rapid amplification of cDNA ends, quantitative polymerase chain reaction (qPCR),  in situ  hybridization, western blotting, RNA interference (RNAi), and histological analyses to assess the potential functions of  Mn-PDHE1  in the sexual development of male  M. nipponense . The full cDNA sequence of  Mn-PDHE1  was 1,614 base pairs long, including a 1,077 base pair open reading frame that encodes 358 amino acids. qPCR analysis revealed the regulatory functions of  PDHE1  in male sexual development in  M .  nipponense  and in the metamorphosis process.  In situ  hybridization and western blot results indicated that  PDHE1  was involved in testis development, and RNAi analysis showed that  PDHE1  positively regulated the expression of insulin-like androgenic gland factor in  M. nipponense . Compared with the cell types in the testes of control prawns, histological analysis showed that the number of sperm was dramatically lower after test subjects were injected with  Mn-PDHE1  dsRNA, whereas the numbers of spermatogonia and spermatocytes were higher. Sperm constituted only 1% of cells at 14 days after injection in the RNAi group. This indicated that knockdown of the expression of  PDHE1  delayed testis development. Thus,  PDHE1  has positive effects on male sexual development in  M .  nipponense . This study highlights the functions of P DHE1  in  M .  nipponense  and its essential roles in the regulation of testis development.

Identification and Characterization of the Pyruvate Dehydrogenase E1 Gene in the Oriental River Prawn, Macrobrachium nipponense