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The study of surface and interfacial science via analytical techniques is a growing interest in the easy mode of diagnosing probe molecules adsorbed on the surface [1]. In that line, Raman spectroscopy is an analytical method that is inefficient to carry the surface analysis employing their weak cross-sectional scattering until the discovery of Surface-Enhanced Raman Scattering (SERS) in late 1970s [2,3]. SERS creates a revolution in many fields, including sensor and biomedical applications [4-6]. It works, when the incident light hits on rough metal surface adsorbed on the probe molecule gets increased Raman signal in a few orders of magnitude [7,8]. The noble metal-based nanomaterials (NMs) such as Ag [9], Au [10], Pt [11], Pd [12], Re [13], Os [14], Ir [15], and RuO2[16] having the remarkable surface plasmon resonance (SPR) nature in the visible region. Many factors that are influencing the enhancement of such SERS signal mainly, wavelength, polarization, and incidence angle of the laser light [17-19]. Besides, the size and shape of nanomaterials was greatly provoking the SERS signal by increasing the hot-spots [20]. Therefore, tuning of such NMs in a controlled way of keeping the inter-particle gap below 5 nm (more hotspots) is a remarkable way of increasing the SERS signal [9]. To increase such hot spots in the NMs using simple methods is the ultimate aim of the researchers to get a high enhancement factor [21-23]. Noble metals such as Ag, Au, and Pd has been well studied earlier [9,24], which are more active contenders for SERS. In that line, Rh NMs is a less explored material as SERS substrate for probe detection [25]. Thus, we have chosen Rh NPs prepared on bio-molecule DNA as scaffold for an active SERS substrate to carry the probe detection.

Engineered Rh Nano-networks on DNA for SERS Applications