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The regeneration of plants from cell and tissue culture is an important and essential component of biotechnology that is required for the genetic manipulation of plants. High frequency regeneration of plants from in vitro cultured tissues and cells are a pre-requisite for successful application of tissue culture and genetic engineering technologies for crop improvement. Culture of explants produces calli that are suitable materials for genetic transformation. Many attempts have been made to enhance the frequency of plant regeneration from brinjal callus and a lot of research programmes have been devoted to investigate the factors affecting plant regeneration. Healthy seeds of brinjal cv. Jhumki were collected from Bangladesh Agricultural Research Institute (BARI). The seeds were then washed thoroughly in running tap water. Special care was taken to avoid all types of injury. The surface sterilization of these seeds was carried out under a Laminar Air Flow Cabinet. The floated seeds were discarded and others were rinsed in 70% ethyl alcohol for one minute, and then thoroughly washed with sterilized distilled water. The alcohol treated seeds were immersed into 0.1% HgCl2 solution for 8-10 minutes, few drops Tween-20 per 100 ml was also added at that time. The seeds were then washed 5-6 times with sterilized distilled water. Sterilized seeds were placed into seed germination medium in Petri dishes. Six seeds were placed in each petridish. The culture was then incubated in dark till the germination of seeds. These were then transferred to 16 hours light for normal seedling growth. MS basal medium with different concentrations and combinations of BAP (0, 2.0, 3.0 and 4.0 mg/l) and NAA (0, 0.1, 0.5 and 1.0 mg/l) were used. Stem segments from each germinated seedling were cut into 2-3 mm pieces using sterilized scalpel under a Laminar Air Flow Cabinet. Six pieces of stem segments were arranged horizontally on each petridish and gently pressed into the surface of the sterilized culture medium with various concentrations and combinations of hormones like NAA and BAP. The petridish was covered and sealed with Para film. Leaf segment from each germinated seedling were cut into small pieces using sterilized scalpel under a Laminar Air Flow Cabinet. Six pieces of leaf segments were arranged on each petridish and gently pressed into the surface of the sterilized culture medium. The Petri dishes were covered and sealed with Para film.

<i>In Vitro</i> Regeneration of Brinjal (<i>Solanum melongena</i> L.) Using Stem and Leaf Explants