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The total fat and fatty acid composition of three commonly consumed small indigenous fish species of Bangladesh, Mola, Amblypharyngodon mola, Jat puti , Puntius sophore and Shar puti, Systomus sarana . Twenty-one types of fatty acids were identified from the extracted oil of these fishes. C16:0 and C18:0 were the predominant saturated fatty acids (SFA), C16:1 ω 7 and C18:1 ω 9 the main monounsaturated fatty acids (MUFA), while C22:6:4 ω 3 (DHA), C20:5 ω 3 (EPA) were the main PUFAs. The total ω 3 was between 4.28% to17.86% and the total ω 6 was between 4.08% to 23.12%. The ω 3 / ω 6 ratio was between to 0.35% to 1.50%. It is evident from this study that A. mola, P. sophore and S. Sarana a re good source of ω 3 and ω 6 PUFA and their regular consumption will contribute to better health. Bangladesh J. Zool. 43 (1): 85-93, 2015

Fatty acid composition of three small indigenous fishes of Bangladesh