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Objective: To evaluate various explants and growth regulators in order to improve in vitro propagation of Stevia rebaudiana through organogenesis. Design/Methodology/Approach: Explants and growth regulators in two different concentrations were evaluated. The explants were nodal segment, axillary bud, and apical meristem; while the growth regulators were benzylaminopurine (BAP) at 1.125 mg L and 3.0 mg L, naphthaleneacetic acid (NAA) at 1.5 mg L and 3.0 mg L, and CIDEF-4 brassinosteroids (BRs) at 1.0 mg L and 1.5 mg L. In total 18 treatments with seven repetitions. Contamination, oxidation, and survival were recorded during induction; while leaf number, regrowth height, and root presence were recorded during multiplication. Results: At the induction stage there was a differential response between explants according to their ontogenetic age; during multiplication, the morphological components showed differences between concentrations of growth regulators and explants, with higher effectiveness when adding BAP to apical meristems. Study Limitations/Implications: Both the origin and the age of explants can induce differential growth while interacting with growth regulators. Findings/Conclusions: Apical meristem explants showed better advantages for in vitro reproduction of S. rebaudiana since they present less contamination and higher survival at the induction stage, even when exhibiting the highest oxidation among explants, which did not influence the decrease in their survival. At the multiplication stage with apical meristem, height, leaf number, and root presence were increased. Values were high when interacting with BAP.

In vitro Organogenesis of Stevia rebaudiana Bert. with Different Explants and Growth Regulators