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Alternaria alternata was isolated from leaf spots of Bael (Aegle marmelos). The fungus was maintained as pure culture in the laboratory. Experiments were done to determine the efficacy of antifungal activity of extracts of selected medicinal plants in vitro. Chirchiri, (Achyranthes aspera), Garlic (Allium sativa), Datura (Datura metal), Lippia nodiflora, Neem (Azadirachta indica) and Tulsi (Ocimum sanctum) were used for the extraction from leaves and cloves etc. Three different concentrations 5, 10 and 15% were used in the culture. The fungitoxic capability was determined by measuring the radial growth among the treated cultures as well as in the control. In the present study, it was observed that extract of clove of garlic at its all the three concentrations viz., 5, 10 and 15 % could inhibit the radial growth of the fungus which was 78.95, 85.05, and 88.54% respectively that was the maximum. This was followed by the extract of neem leaves that was 75.77, 82.35 and 87.12% respectively. It was further observed that leaf extract of Chirchiri had the minimum inhibitory activity at all the concentrations used, that was 63.73, 68.14 and 72.52% only. It was further observed that leaf extract of Ocimum sanctum inhibited the radial growth at all its three concentrations which was next to neem leaf extract that was 75.32, 81.07, and 85.48% respectively. This was followed by leaf extract of Lippia that was 74.53, 80.62 and 84.46 respectively. Therefore, leaf and clove extracts of different medicinal plants revealed, antifungal activities at different concentrations with different quantum of action.

indian journal of scientific research

In Vitro Determination Of Efficacy Of Extracts Of Selected Medicinal Plants Against Alternaria alternata Isolated From Leaf Spot Of Bael (Aegle marmelos)