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This review documents some new advances in the field of Veterinary medicine with specific focus on the exciting research area of myo-fascia. It presents some of the latest findings in structure as well as function of these interconnected tissues, as well as highlighting the effects and benefits of treatment for such underlying issues as regional stiffness affecting performance, and the role of myofascial kinetic lines. It likewise addresses some of the new and non-invasive techniques such as accelerometry, multi-frequency bioimpedance and acoustic myography that have emerged over recent years, and illustrates how they can be readily adopted in the Veterinary clinic to assess muscle imbalance and injury as well as to direct such treatment as myofascial release therapy, acupuncture and proprioception and to follow rehabilitation. Copyright: © 2018 Science Publishing Group This open access article is distributed under the terms of the Creative Commons Attribution NonCommercial License. Applied Myo-Fascial Advances in Veterinary Medicine and Practice. Science Publishing Group Copyright 2018 www.spg.ltd 3 Introduction: With new technology come new possibilities of detecting, diagnosing and even treating injuries, illnesses and diseases, not only in the field of human medicine, but also that of veterinary medicine. However, new advances can also come with new insights, improvements and understanding of existing techniques or even more careful and detailed dissection procedures (Elbrønd et al 2015; Harrison et al 2018; Elbrønd & Schultz, 2015; Elbrønd & Schultz, 2018; Jensen et al 2018; Chavers et al 2018).